Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CTCF

Cell type

Cell type Class
Epidermis
Cell type
MDA-MB-435
Primary Tissue
Skin
Tissue Diagnosis
Melanoma

Attributes by original data submitter

Sample

source_name
Mitotic pT431 CTCF ChIP-seq
cell line
MDA-MB-435 melanoma cells
cell cycle stage
Mitosis (nocodazole-arrested)
chip antibody
rabbit anti-pT431 CTCF (custom)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP_protocol.pdf Libraries were constructed with the NEBNext Ultra II DNA Library Prep Kit for Illumina at the Indiana University Center for Genomics and Bioinformatics

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
29803966
Reads aligned (%)
188.8
Duplicates removed (%)
3.2
Number of peaks
445 (qval < 1E-05)

hg38

Number of total reads
29803966
Reads aligned (%)
190.9
Duplicates removed (%)
3.0
Number of peaks
584 (qval < 1E-05)

Base call quality data from DBCLS SRA