Antigen

Antigen Class

TFs and others

Antigen

CTCF

Cell type

Cell type Class

Epidermis

Cell type

MDA-MB-435

Primary Tissue

Skin

Tissue Diagnosis

Melanoma

Sample

source_name

Asynchronous pan-CTCF ChIP-seq

cell line

MDA-MB-435 melanoma cells

cell cycle stage

Mixed (asynchronous)

chip antibody

rabbit anti-CTCF (Millipore 07-729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP_protocol.pdf Libraries were constructed with the NEBNext Ultra II DNA Library Prep Kit for Illumina at the Indiana University Center for Genomics and Bioinformatics

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

26103059

Reads aligned (%)

96.1

Duplicates removed (%)

5.3

Number of peaks

17666 (qval < 1E-05)

hg19

Number of total reads

26103059

Reads aligned (%)

95.2

Duplicates removed (%)

5.5

Number of peaks

18000 (qval < 1E-05)