Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC Cyt49

NA

NA

Sample

source_name

hESC

cell line

CyT49

cell types

Undifferentiated hESCs

antibody

H3K4me1, Abcam ab8895

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq protocol can be found from http://bioinformatics-renlab.ucsd.edu/RenLabLibraryProtocolV1.pdf. GRO-seq experiments were performed according to previous publication (Wang, D. et al. Reprogramming transcription by distinct classes of enhancers functionally defined by eRNA. Nature 474, 390-4 (2011).).  Libraries were prepared according to Illumina's instruction or cited literatures.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

38283300

Reads aligned (%)

97.7

Duplicates removed (%)

2.7

Number of peaks

4850 (qval < 1E-05)

hg19

Number of total reads

38283300

Reads aligned (%)

97.3

Duplicates removed (%)

3.5

Number of peaks

4669 (qval < 1E-05)