Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Epidermis

Cell type

Epidermis

MeSH Description

The external, nonvascular layer of the skin. It is made up, from within outward, of five layers of EPITHELIUM: (1) basal layer (stratum basale epidermidis); (2) spinous layer (stratum spinosum epidermidis); (3) granular layer (stratum granulosum epidermidis); (4) clear layer (stratum lucidum epidermidis); and (5) horny layer (stratum corneum epidermidis).

Sample

source_name

IFE_input DNA_ChIP-Seq

strain background

CD-1

gender

female

age

P52-60

tissue

back skins

sorting markers

integrin α6hi and CD34- and ScaI-

cell type

intefollicular epidermal cells (IFE)

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-Seq: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303). RNA-Seq: Illumina TruSeq™ RNA kit (Catalog # RS-930-20 01)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

105756748

Reads aligned (%)

67.7

Duplicates removed (%)

27.2

Number of peaks

157 (qval < 1E-05)

mm9

Number of total reads

105756748

Reads aligned (%)

67.5

Duplicates removed (%)

27.2

Number of peaks

231 (qval < 1E-05)