Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Epidermis
Cell type
Hair follicle stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
HFSC_input DNA_ChIP-Seq
strain background
CD-1
gender
female
age
P52-60
tissue
back skins
sorting markers
integrin α6hi and CD34+ and ScaI-
cell type
hair follicle stem cells (HFSCs)
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-Seq: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303). RNA-Seq: Illumina TruSeq™ RNA kit (Catalog # RS-930-20 01)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
55130206
Reads aligned (%)
71.8
Duplicates removed (%)
10.9
Number of peaks
266 (qval < 1E-05)

mm9

Number of total reads
55130206
Reads aligned (%)
71.8
Duplicates removed (%)
10.9
Number of peaks
238 (qval < 1E-05)

Base call quality data from DBCLS SRA