Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Pluripotent stem cell

Cell type

P19 derived neural cells

NA

NA

Sample

source_name

day 3 neuronal cells

chip antibody

IgG

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were crosslinked with 5% formaldehyde for 5 mins. Nuclei were extracted, chromatin sonicated, and ChIP was performed using protein G agarose using standard procedures. NEB ultra DNA library prep kit

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

18165825

Reads aligned (%)

67.7

Duplicates removed (%)

53.5

Number of peaks

319 (qval < 1E-05)

mm9

Number of total reads

18165825

Reads aligned (%)

67.5

Duplicates removed (%)

53.7

Number of peaks

278 (qval < 1E-05)