Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
P19
Tissue
Embryo
Disease
Teratocarcinoma; Embryonal Carcinoma

Attributes by original data submitter

Sample

source_name
undifferentiated P19 EC cells
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with 5% formaldehyde for 5 mins. Nuclei were extracted, chromatin sonicated, and ChIP was performed using protein G agarose using standard procedures. NEB ultra DNA library prep kit

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
47509164
Reads aligned (%)
89.0
Duplicates removed (%)
8.9
Number of peaks
643 (qval < 1E-05)

mm9

Number of total reads
47509164
Reads aligned (%)
88.9
Duplicates removed (%)
9.0
Number of peaks
659 (qval < 1E-05)

Base call quality data from DBCLS SRA