GSM3315626: GDC-0927-45m ERa; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
cell line
cell line
MCF-7
ligand
GDC-0927
time
45min
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed by incubation in 11% formaldehyde solution for 15 minutes at room temperature. Fixation was stopped by the addition of a 2.5 M glycine solution. Cells were washed in a 0.5% Igepal-PBS solution prior to snap-freezing. Active Motif (Carlsbad, CA) performed chromatin isolation, immunoprecipitation reactions, library generation and sequencing. ER ChIP reactions were conducted using 30 μg MCF7 breast cancer cell line chromatin and 4 μg of ER alpha antibody (sc-543, Santa Cruz Biotechnology, Dallas, TX). The ChIP reactions also contained a drosophila chromatin spike in for the normalization of the sequencing data. Libraries were prepared from each ER ChIP reaction, as well as an input/control DNA sample, and single-end sequencing data were generated on the Illumina platform