GSM3314515: H3K4me3 ChiPSeq Crohn's 3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Digestive tract
Cell type
Intestinal epithelial cells
NA
NA
Attributes by original data submitter
Sample
source_name
Ileal endoscopic biopsy
cell type
Intestinal epithelial cells
diagnosis
Newly-diagnosed Crohn's Disease
age
12-18 years
chip antibody
Histone H3 lysine 4 trimethylation
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Fresh formaldehyde was added to each IEC sample for a final concentration of 1% and mixed end over end at room temperature for 10min to crosslink proteins to DNA. Cells were lysed with a Triton X-100 and Igepal buffer (0.25% Triton C-100, 0.5% Igepal, 10% glycerol, 1mM EDTA, 140mM NaCl, 50mM Hepes). Nuclei were then washed and chromatin sheared in a 0.1%SDS in TE buffer using a S220 Covaris Sonicator to 150-500 bp size. Immunoprecipitation was carried out with a SX-8G IP-STAR robot and an antibody optimized for histone H3 lysine 4 trimethylation (H3K4me3) as described previously. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit. Sequencing was performed using Illumina HiSeq 2500.