GSM3312819: H3K27ac ChIP seq in SEM cells treated with 2uM EPZ-5676; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Blood
Cell type
SEM
Primary Tissue
Blood
Tissue Diagnosis
Leukemia
Attributes by original data submitter
Sample
source_name
H3K27ac_DOT1Li
cell line
Leukemia cell line (SEM)
treatment
EPZ-5676 at 2uM for 7 days
cell type
Paediatric pro B-cell line derived from ALL with t(4;11)(q21;q23) translocation
chip antibody
H3K27ac (Diagenode C15410196)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were washed with PBS and fixed in either 1% FA for 10 minutes (histones) or 2mM DSG for 30 minutes and 1% FA for 30 minutes (transcription factors). Cells were sonicated to give fragments of 100-300bp followed by IP. For ChIP-rx fixed SEM cells were mixed at a 4:1 ratio with fixed Drosophila S2 cells prior to sonication. DNA libraries were made using the NEBnext ultra DNA library preparation kit for Illumina (Cat no. E7370).