GSM3310643: dI5: S5 input DNA rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
HEK293
cell line
HEK293
tissue
Human embryonic kidney cells
chip antibody
none
inhibitor
Flavopiridol
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
HEK293 cells were cross-linked using formaldehyde, washed with cold PBS twice, and scraped in a small amount of PBS. The cells were collected and lysed using ChIP cell and nuclei lysis buffers (refer to the manuscript associated with the submission). The chromatins were sheared in nuclei lysis buffer by sonication. S2 Pol II antibody was used for immunoprecipitation of the chromatin associated with the target protein. Illumina libraries were prepared using a Beckan-Coulter SPRIworks system and sequenced on an Illumina HiSeq2000 using a 40nt single end read.