Antigen

Antigen Class

TFs and others

Antigen

MLLT3

Cell type

Cell type Class

Blood

Cell type

CD34+

NA

NA

Sample

source_name

umbilical cord blood

cell type

CD34+ cells

transduction

HSPC-MA9

chip antibody

AF9 C-terminus (Bethyl 597A)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. 9 ng of ChIP DNA as quantitated by fluorometry was used. Electrophoretic fraction of 150 bp was processed through subsequent enzymatic treatments of end-repair, dA-tailing, and ligation to adapters as in Illumina's "TruSeq DNA Sample Preparation Guide" (part # 15005180 Rev. C). Adapter-ligated library was completed by limited-cycle PCR with Illumina PE primers (12 cycles). The resulting purified DNA library was applied to an Illumina flow cell for cluster generation (TruSeq cluster generation kit v5) and sequenced on the Genome Analyzer IIx with SBS TruSeq v5 reagents by following manufacturer's protocols.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg38

Number of total reads

21881549

Reads aligned (%)

78.7

Duplicates removed (%)

16.2

Number of peaks

561 (qval < 1E-05)

hg19

Number of total reads

21881549

Reads aligned (%)

77.9

Duplicates removed (%)

17.8

Number of peaks

798 (qval < 1E-05)