Antigen

Antigen Class

TFs and others

Antigen

EP300

Cell type

Cell type Class

Blood

Cell type

Kasumi-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

Kasumi-1 Cells

cell line

Kasumi-1

cell type

Acute Myeloid Leukemia

chip antibody

P300 (Santa Cruz sc-585)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

SimpleChIP Enzymatic Chromatin IP kit (Cell Siganling Technology) was used for chromatin immunoprecipitation per manufacturers instructions. Libraries were prepared using E7645S NEBNext(r) Ultra(tm) II DNA Library Prep Kit for Illumina® accoring to manufacturers instructions.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg19

Number of total reads

27014772

Reads aligned (%)

83.1

Duplicates removed (%)

13.2

Number of peaks

22359 (qval < 1E-05)

hg38

Number of total reads

27014772

Reads aligned (%)

84.5

Duplicates removed (%)

12.3

Number of peaks

22263 (qval < 1E-05)