Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
CAP600 H3K4me3
cell line
MCF-7
cell type
Mammary epithelial cell line
treatment
Cold atmospheric plasma (CAP)
chip antibody
Histone H3K4me3 Polyclonal Antibody
chip antibody vendor
Signalway Antibody

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted using DNA Prep. Kit construction of library was performed using NEBNext® UltraTM DNA Library Prep Kit for Illumina (New England Biolabs, UK) according to the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
20883599
Reads aligned (%)
98.4
Duplicates removed (%)
2.1
Number of peaks
22561 (qval < 1E-05)

hg19

Number of total reads
20883599
Reads aligned (%)
97.9
Duplicates removed (%)
2.2
Number of peaks
22276 (qval < 1E-05)

Base call quality data from DBCLS SRA