GSM3305860: Patient 4-CD45RApos; Homo sapiens; DNase-Hypersensitivity
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
DNase-seq
Antigen
DNase-Seq
Cell type
Cell type Class
Blood
Cell type
CD34+ cells
NA
NA
Attributes by original data submitter
Sample
source_name
CD34 positive AML cells from peripheral blood
condition
AML
Sequenced DNA Library
library_strategy
DNase-Hypersensitivity
library_source
GENOMIC
library_selection
DNase
library_construction_protocol
Cells were thawed in a water bath at 22C for 2 minutes, prior to addition of 120ul of DNaseI buffer consisting of 60nM KCL, 15 mM NaCl and 5 mM MgCL2. Digests were performed by adding 150 uL of DNaseI buffer equilibrated at 22C, containing 0.4% Nonidet P40 (or IGEPAL CA-630), 2 mM CaCl2, and either 1.5, 3 or 5 units/mL of Worthington DPFF DNase I. The permeabilized cells were digested for exactly 3 minutes at 22C, with occasional tapping to keep tubes in suspension, and lysed by addition of 300uL of 0.3 M NA Acetate, 10 mM EDTA pH 7.4, 1% SDS and 1 mg/Ml proteinase K. DNA was purified, size-selected, and processed for DNA-Seq with a MicroPlex library preparation kit v2 (Diagenode)