Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
N6AMT1

Cell type

Cell type Class
Prostate
Cell type
PC-3M
NA
NA

Attributes by original data submitter

Sample

source_name
PC-3M cells
cell line
PC-3M
cell type
prostate cancer cells
phenotype
AR-independent
treatment
siRNA_KMT9a
chip antibody
anti-KMT9a, #27630, lot 20062017, Schüle Lab

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Metzger, E., et al. Phosphorylation of histone H3 at threonine 11 establishes a novel chromatin mark for transcriptional regulation. Nat Cell Biol 10, 53-60 (2008). Libraries were prepared from immunoprecipitated DNA according to standard methods. ChIP-seq libraries were sequenced using a HiSeq 2000 (Illumina) and mapped to the hg19 reference genome using STAR

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
18013712
Reads aligned (%)
74.6
Duplicates removed (%)
15.0
Number of peaks
396 (qval < 1E-05)

hg38

Number of total reads
18013712
Reads aligned (%)
75.0
Duplicates removed (%)
14.9
Number of peaks
378 (qval < 1E-05)

Base call quality data from DBCLS SRA