Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Arntl

Cell type

Cell type Class
Liver
Cell type
Liver
MeSH Description
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Attributes by original data submitter

Sample

source_name
saline-treated Liver
agent
saline
tissue
Liver
strain
C57BL/6
age
adult
Sex
male
chip-antibody
BMAL1 Perelis et al., 2015
protocol
Barish and Tangirala, 2013

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using NEBNext Ultra II DNA Library Preparation kits (NEB) according to the manufacturer's instructions. Quality of the library and concentration were determined by Bioanlayzer using the High Sensitivity chip (Agilent) and qPCR-based quantification (NEBNext Library Quant Kit), respectively.

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
29690431
Reads aligned (%)
75.9
Duplicates removed (%)
15.7
Number of peaks
9617 (qval < 1E-05)

mm9

Number of total reads
29690431
Reads aligned (%)
75.8
Duplicates removed (%)
15.7
Number of peaks
9608 (qval < 1E-05)

Base call quality data from DBCLS SRA