Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H4K12ac

Cell type

Cell type Class
Kidney
Cell type
hTERT-HME1
Primary Tissue
Breast
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
hTert-HME1 cell line
cell line
hTert-HME1
treatment
shHAT1
replicate
3
chip antibody
h4k12ac -- abcam cat# ab46983

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
1% formaldehyde crosslinking, followed by isolation of nuclei, lysis in RIPA buffer, then sonication with Branson and Bioruptor Pico, clarification of extract, then immunoprecipitation with specific antibodies Illumina Truseq

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
47168784
Reads aligned (%)
98.3
Duplicates removed (%)
5.7
Number of peaks
1679 (qval < 1E-05)

hg19

Number of total reads
47168784
Reads aligned (%)
97.3
Duplicates removed (%)
6.1
Number of peaks
521 (qval < 1E-05)

Base call quality data from DBCLS SRA