GSM3301565: shHAT1 A7 h4k12ac ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H4K12ac
Cell type
Cell type Class
Kidney
Cell type
hTERT-HME1
Primary Tissue
Breast
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
hTert-HME1 cell line
cell line
hTert-HME1
treatment
shHAT1
replicate
1
chip antibody
h4k12ac -- abcam cat# ab46983
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
1% formaldehyde crosslinking, followed by isolation of nuclei, lysis in RIPA buffer, then sonication with Branson and Bioruptor Pico, clarification of extract, then immunoprecipitation with specific antibodies Illumina Truseq