Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
Nucleus Accumbens
MeSH Description
Collection of pleomorphic cells in the caudal part of the anterior horn of the LATERAL VENTRICLE, in the region of the OLFACTORY TUBERCLE, lying between the head of the CAUDATE NUCLEUS and the ANTERIOR PERFORATED SUBSTANCE. It is part of the so-called VENTRAL STRIATUM, a composite structure considered part of the BASAL GANGLIA.

Attributes by original data submitter

Sample

source_name
Nucleus Accumbens
tissue
NAc
development stage
6-8 weeks
antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. RNA was isolated from mouse nucleus accumbens for mRNA sequencing by following illumina RNA seq kit protocol. Library was prepared following illumina protocol. All RNA samples were pooled and sequenced in the same lane. All ChIP-seq were pooled and sequenced in the same lane.

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
5081902
Reads aligned (%)
65.5
Duplicates removed (%)
38.8
Number of peaks
116 (qval < 1E-05)

mm9

Number of total reads
5081902
Reads aligned (%)
65.3
Duplicates removed (%)
39.3
Number of peaks
123 (qval < 1E-05)

Base call quality data from DBCLS SRA