Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Embryonic stem cells
tissue
Embryonic stem cells
chip antibody
None
cell type
WT R1 ESCs
culture conditions
Serum/LIF +2i

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Libraries were prepared for Illumina sequencing using 5 ng of ChIP material with a Kapa Hyper Prep Kit (Kapa Biosystems), according to the manufacturer's instructions

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
41790621
Reads aligned (%)
94.2
Duplicates removed (%)
18.5
Number of peaks
389 (qval < 1E-05)

mm9

Number of total reads
41790621
Reads aligned (%)
94.1
Duplicates removed (%)
18.5
Number of peaks
383 (qval < 1E-05)

Base call quality data from DBCLS SRA