Antigen

Antigen Class

Histone

Antigen

H3R2me2

Cell type

Cell type Class

Pluripotent stem cell

Cell type

NT2-D1

Tissue

testis

Lineage

inner cell mass

Description

malignant pluripotent embryonal carcinoma (NTera-2), "The NTERA-2 cl.D1 cell line is a pluripotent human testicular embryonal carcinoma cell line derived by cloning the NTERA-2 cell line." - ATCC. (PMID: 6694356)

Sample

source_name

NT2D1_CT_H3R2me2a_-Inh

cell line

NT2/D1

cell type

embryonal carcinoma cell line

cell status

pluripotent

genotype/variation

CRISPR/Cas9 control NT2/D1 cells

chip antibody

rat monoclonal generated in the laboratory

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were crosslinked in the presence of 1% formaldehyde. Cell lysates were sonified and Protein-DNA complexes were enriched by immunoprecipitation with mentioned antibodies. Libraries were prepared according to the manufacturer's protocol (Diagenode, MicroPlex Library Preparation Kit)

Sequencing Platform

instrument_model

Illumina HiSeq 1500

hg38

Number of total reads

35631226

Reads aligned (%)

97.9

Duplicates removed (%)

3.2

Number of peaks

925 (qval < 1E-05)

hg19

Number of total reads

35631226

Reads aligned (%)

97.1

Duplicates removed (%)

4.1

Number of peaks

1008 (qval < 1E-05)