Antigen

Antigen Class

Histone

Antigen

H3R2me2

Cell type

Cell type Class

Bone

Cell type

U2OS

Tissue

bone

Lineage

mesoderm

Description

osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Sample

source_name

U2OS_KO_H3R2me2a

cell line

U2OS

cell type

bone osteosarcoma

cell status

differentiated

genotype/variation

CRISPR/Cas9-mediated deletion of PRMT6 inU2OS cells

chip antibody

rat monoclonal generated in the laboratory

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were crosslinked in the presence of 1% formaldehyde. Cell lysates were sonified and Protein-DNA complexes were enriched by immunoprecipitation with mentioned antibodies. Libraries were prepared according to the manufacturer's protocol (Diagenode, MicroPlex Library Preparation Kit)

Sequencing Platform

instrument_model

Illumina HiSeq 1500

hg38

Number of total reads

63294331

Reads aligned (%)

92.4

Duplicates removed (%)

5.7

Number of peaks

2466 (qval < 1E-05)

hg19

Number of total reads

63294331

Reads aligned (%)

91.6

Duplicates removed (%)

7.1

Number of peaks

2374 (qval < 1E-05)