GSM3270930: CBX4-/- hMSC, sample as input for H3K4me3 ChIP assay [Input-KO-1]; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Others
Cell type
Mesenchymal stem cells
NA
NA
Attributes by original data submitter
Sample
source_name
CBX4-/- hMSC
cell type
MSC
passages
p4
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total RNA was extracted with the RNeasy Mini Kit (Qiagen). To obtain the genomic DNA fragments of interest for ChIP-seq, the cells were crosslinked and lysed, and then the chromatin was sheared into fragments using sonication. Then samples were incubated with anti-H3K4me3 antibody (Abcam, ab8050, lot#GR3190162-1) or anti-FLAG antibody overnight. The DNA was de-crosslinked and extracted on the next day. 1.5 μg of total RNA was used to construct RNA sequencing libraries by NEB Next Ultra RNA Library Prep Kit (NEB) for Illumina following the manufacturer's recommended protocol. The ChIP-sequencing library and copy number variation analysis sequencing library were constructed by NEB Next Ultra DNA Library Prep Kit (NEB) for Illumina according to the manufacturer's instructions.