GSM3263336: HCC1806 BRD2 BAZ2BsiRNA 48h; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
BRD2
Cell type
Cell type Class
Breast
Cell type
HCC1806
Primary Tissue
Breast
Tissue Diagnosis
Carcinoma Squamous Cell
Attributes by original data submitter
Sample
source_name
HCC1806 breast carcinoma cell line
cell line
HCC1806
treatment
BAZ2BsiRNA 48h
culture media
RPMI supplemented with 10% FBS
library preparation kit
KAPA Hyper Prep
chip antibody
BRD2 Bethyl Laboratories A302-583A
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell lysates were sonicated resulting in an average chromatin fragment size of ~300 bp and immunoprecipiated with the indicated antibodies. All libraries were constructed using KAPA Hyper Prep kits with Illumina TruSeq adapters, 18 cycles of amplification and dual SPRI size selection post-amplification