GSM3262118: Wild type Input DNA; Drosophila melanogaster; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage
Attributes by original data submitter
Sample
source_name
Input DNA from normal S2 cells
cell line
S2
cell origin
Embryonic cell line
passages
15-20
genotype/variation
wild type
chip antibody
None
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated cells and DNA complexes were isolated with antibody. ChIP-DNA libraries were produced using the NEBNext Ultra II DNA Library Prep Kit (NEB E7645L) and NEBnext Multiplex Oligos for Illumina Dual Index Primers (NEB E7600S), using provided protocols with 10ng of fragmented ChIP DNA