ChIP extraction done 1% SDS lysis buffer followed by Immunoprecipitation in a low detergent buffer (dilution 1/10 of original lysis buffer) using lab protocol on a sciclone ngs liquid handler with lab developed protocols with Santa Cruz HC-20 and the monoclonal ER Ab10 antibodies libraries generated using standard protocol, end repair, AT overhang, oligo ligation, pippin prep size selection, PCR amplification, pippin prep size selection, Ethanol salt precipitation