GSM3261522: MtbH37Ra infected (4hrs) THP-1 H3K4me3 ChIPSeq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Blood
Cell type
THP-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous
Attributes by original data submitter
Sample
source_name
MtbH37Ra infected (4hrs) THP-1 cells
cell type
THP-1 differentiated macrophage
infected with
Mtb H37Ra avirulent strain
passages
Passage 9
chip antibody
H3K4me3 (Abcam ChIP grade,ab8580)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were dislodged using Accutase (Himedia). Chromatin Immunoprecipitation (ChIP) was done using the Diagenode Ideal Chip seq kit and H3K4me3 specific antibody. library preparation was performed according to the manufacturer's protocol for the Illumina Hiseq 2000 platform