Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NFKB1

Cell type

Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
ChIP-seq_NFƘB_Act.5 h
subject status
healthy donor
tissue
peripheral blood
cell type
primary naive CD4 T cells
activation
Act. 5 h with anti-CD3 and -CD28 antibodies
chip antibody
NFƘB p50 Cell Signaling #12540

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was immunoprecipitated with antibody and tagmented with nextera transposome (illumina) according to the ChIPmentation protocol in Schmidl et al (PBMD: 26280331). Libraries were prepared by chipmentation.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
17362455
Reads aligned (%)
88.3
Duplicates removed (%)
8.7
Number of peaks
1627 (qval < 1E-05)

hg38

Number of total reads
17362455
Reads aligned (%)
90.4
Duplicates removed (%)
7.5
Number of peaks
1495 (qval < 1E-05)

Base call quality data from DBCLS SRA