Antigen

Antigen Class

TFs and others

Antigen

NFATC1

Cell type

Cell type Class

Blood

Cell type

CD4+ T cells

NA

NA

Sample

source_name

ChIP-seq_NFAT2_Act.5 h

subject status

healthy donor

tissue

peripheral blood

cell type

primary naive CD4 T cells

activation

Act. 5 h with anti-CD3 and -CD28 antibodies

chip antibody

NFAT2 Cell Signaling #8032

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was immunoprecipitated with antibody and tagmented with nextera transposome (illumina) according to the ChIPmentation protocol in Schmidl et al (PBMD: 26280331). Libraries were prepared by chipmentation.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

36321322

Reads aligned (%)

90.7

Duplicates removed (%)

45.4

Number of peaks

1119 (qval < 1E-05)

hg19

Number of total reads

36321322

Reads aligned (%)

89.8

Duplicates removed (%)

46.7

Number of peaks

942 (qval < 1E-05)