Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NFATC2

Cell type

Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
ChIP-seq_NFAT1_Act.5 h_without_CD28
subject status
healthy donor
tissue
peripheral blood
cell type
primary naive CD4 T cells
activation
Act. 5 h with anti-CD3 antibody
chip antibody
NFAT1 Cell Signaling #5861

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was immunoprecipitated with antibody and tagmented with nextera transposome (illumina) according to the ChIPmentation protocol in Schmidl et al (PBMD: 26280331). Libraries were prepared by chipmentation.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
33161507
Reads aligned (%)
78.4
Duplicates removed (%)
13.7
Number of peaks
2522 (qval < 1E-05)

hg38

Number of total reads
33161507
Reads aligned (%)
80.5
Duplicates removed (%)
12.4
Number of peaks
2728 (qval < 1E-05)

Base call quality data from DBCLS SRA