Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
Corpus Callosum
MeSH Description
Broad plate of dense myelinated fibers that reciprocally interconnect regions of the cortex in all lobes with corresponding regions of the opposite hemisphere. The corpus callosum is located deep in the longitudinal fissure.

Attributes by original data submitter

Sample

source_name
O4+ cells
tissue
Cortex and Corpus callosum
age
Postnatal day 7
chip antibody
Rabbit IgG

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and Chd7-DNA and Chd8-DNA complexes were isolated with O4 antibody. The ChIP-seq libraries were prepared using TruSeq ChIP library preparation kit (Illumina ip-202-1012)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
49408247
Reads aligned (%)
19.1
Duplicates removed (%)
54.7
Number of peaks
77 (qval < 1E-05)

mm9

Number of total reads
49408247
Reads aligned (%)
19.1
Duplicates removed (%)
54.8
Number of peaks
59 (qval < 1E-05)

Base call quality data from DBCLS SRA