Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Prostate
Cell type
PC-3
Primary Tissue
Prostate
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
PC-3 cells
cell line
PC-3 prostate cancer cells
antibody
rabbit antiserum against AR
treatment
10 nM R1881
genotype/variation
wild-type AR

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and AR-DNA complexes were isolated with 1 µg of antibody. ChIP-Seq libraries were prepared for sequencing using standard Illumina protocols, as utilized at the Gene Core in EMBL, Heidelberg, Germany

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
213074598
Reads aligned (%)
65.7
Duplicates removed (%)
32.0
Number of peaks
42033 (qval < 1E-05)

hg38

Number of total reads
213074598
Reads aligned (%)
67.5
Duplicates removed (%)
30.8
Number of peaks
43017 (qval < 1E-05)

Base call quality data from DBCLS SRA