GSM1308233: HEK293 AR-K2R R1881 rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
AR
Cell type
Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
Isogenic HEK293 Cells
cell line
Human Embryonal Kidney Cells HEK-293
antibody
rabbit antiserum against AR
treatment
10 nM R1881
treatment time
40 min
genotype/variation
SUMOylation-deficient AR
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and AR-DNA complexes were isolated with 1 µg of antibody. ChIP-Seq libraries were prepared for sequencing using standard Illumina protocols, in Beijing Genomic institute (BGI)(HongKong, China).