Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
Isogenic HEK293 Cells
cell line
Human Embryonal Kidney Cells HEK-293
antibody
rabbit antiserum against AR
treatment
10 nM R1881
treatment time
40 min
genotype/variation
wild-type AR

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and AR-DNA complexes were isolated with 1 µg of antibody. ChIP-Seq libraries were prepared for sequencing using standard Illumina protocols, in Beijing Genomic institute (BGI)(HongKong, China).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
25759385
Reads aligned (%)
89.7
Duplicates removed (%)
9.5
Number of peaks
55622 (qval < 1E-05)

hg38

Number of total reads
25759385
Reads aligned (%)
90.6
Duplicates removed (%)
8.9
Number of peaks
55740 (qval < 1E-05)

Base call quality data from DBCLS SRA