Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
CD4 T cells
strain
C57Bl/6Ncr
genotype
ThpokBio BirA
immunoprecipitation
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was treated with proteinase K and RNase (both at 0.2mg/ml) before purification using the QIAquick PCR purification kit (Qiagen) Accel-NGS 2S DNA reagent (Swift) Libraries were sequenced (75bp single-ended reads) on a NextSeq sequencer (Illumina)

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
37271733
Reads aligned (%)
96.3
Duplicates removed (%)
16.5
Number of peaks
376 (qval < 1E-05)

mm9

Number of total reads
37271733
Reads aligned (%)
96.0
Duplicates removed (%)
16.4
Number of peaks
464 (qval < 1E-05)

Base call quality data from DBCLS SRA