Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
Macrophages
MeSH Description
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Attributes by original data submitter

Sample

source_name
Monocyte-derived macrophages
donor id
MAC310
cell type
Monocyte-derived macrophages
treatment
S
chip antibody
H3K27ac

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA from macrophages was extracted using the DNeasy Blood and Tissue Kit (Qiagen). We collected ChIP-seq data for the H3K27ac histone mark in non-infected and Salmonella-infected macrophages as previously described (Pacis et al., 2015).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
59659025
Reads aligned (%)
90.7
Duplicates removed (%)
35.4
Number of peaks
27208 (qval < 1E-05)

hg19

Number of total reads
59659025
Reads aligned (%)
90.3
Duplicates removed (%)
35.8
Number of peaks
27087 (qval < 1E-05)

Base call quality data from DBCLS SRA