Curated Sample Data


Genome
dm3
Antigen Class
Input control
Antigen
Input control
Cell type Class
Adult
Cell type
Ovary

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
overies
strain background
w1118
chip antibody
sc-2025, Santa Cruz Biotechnology

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ovaries dissected from fly strains were fixed in 1% PFA for 10 min at RT, homogenized using a pestle in 300 ml of RIPA buffer, and sonicated with Bioruptor (Diagenode).At least 100 ovaries were used per one IP. Immunoprecipitations were carried out with 5 µg antibody overnight at 4°C in a rotating wheel. ChIP libraries were prepared for Illumina NextSeq 500 using NEBNext ChIP-Seq DNA Sample Prep Master Mix Set for Illumina (NEB E6240) and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (NEB E7335) according to the manufacturer's protocols.

Platform Information


instrument_model
NextSeq 500

External Database Query

Logs in read processing pipeline


Number of total reads
25668972
Reads aligned (%)
100.0
Duplicates removed (%)
24.1
Number of peaks
2508 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA