Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Act5C

Cell type

Cell type Class
Adult
Cell type
Ovary
NA
NA

Attributes by original data submitter

Sample

source_name
overies
strain background
w1118
chip antibody
AC-15 (A1978)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ovaries dissected from fly strains were fixed in 1% PFA for 10 min at RT, homogenized using a pestle in 300 ml of RIPA buffer, and sonicated with Bioruptor (Diagenode).At least 100 ovaries were used per one IP. Immunoprecipitations were carried out with 5 µg antibody overnight at 4°C in a rotating wheel. ChIP libraries were prepared for Illumina NextSeq 500 using NEBNext ChIP-Seq DNA Sample Prep Master Mix Set for Illumina (NEB E6240) and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (NEB E7335) according to the manufacturer's protocols.

Sequencing Platform

instrument_model
NextSeq 500

dm3

Number of total reads
7371881
Reads aligned (%)
63.1
Duplicates removed (%)
36.9
Number of peaks
3540 (qval < 1E-05)

dm6

Number of total reads
7371881
Reads aligned (%)
62.4
Duplicates removed (%)
37.5
Number of peaks
3552 (qval < 1E-05)

Base call quality data from DBCLS SRA