Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Adult
Cell type
Ovary
NA
NA

Attributes by original data submitter

Sample

source_name
overies
strain background
mal-d[delta]7
chip antibody
4H8; ab5408, Abcam

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ovaries dissected from fly strains were fixed in 1% PFA for 10 min at RT, homogenized using a pestle in 300 ml of RIPA buffer, and sonicated with Bioruptor (Diagenode).At least 100 ovaries were used per one IP. Immunoprecipitations were carried out with 5 µg antibody overnight at 4°C in a rotating wheel. ChIP libraries were prepared for Illumina NextSeq 500 using NEBNext ChIP-Seq DNA Sample Prep Master Mix Set for Illumina (NEB E6240) and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (NEB E7335) according to the manufacturer's protocols.

Sequencing Platform

instrument_model
NextSeq 500

dm6

Number of total reads
8793811
Reads aligned (%)
100.0
Duplicates removed (%)
59.2
Number of peaks
8289 (qval < 1E-05)

dm3

Number of total reads
8793811
Reads aligned (%)
100.0
Duplicates removed (%)
58.3
Number of peaks
8738 (qval < 1E-05)

Base call quality data from DBCLS SRA