Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RAD21

Cell type

Cell type Class
Muscle
Cell type
RH-4
NA
NA

Attributes by original data submitter

Sample

source_name
RH4
cell line
RH4
antibody
Rad21 (Santa Cruz, ChIP Grade ab992)
enrichment target
RAD21
target function
Cohesin Complex
treatment
Entinostat, 1 µM, 6 hours

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted from ChIP-seq samples prepared following the Active Motif ChIP-IT High Sensitivity kit protocol. ATAC-seq DNA purification was performed by column (MiniElute PCR purification kit, Qiagen). Standard Illumina barcodes were introduced during library preparation

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
44547331
Reads aligned (%)
80.8
Duplicates removed (%)
15.9
Number of peaks
36308 (qval < 1E-05)

hg38

Number of total reads
44547331
Reads aligned (%)
82.8
Duplicates removed (%)
14.3
Number of peaks
36715 (qval < 1E-05)

Base call quality data from DBCLS SRA