Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SOX8

Cell type

Cell type Class
Muscle
Cell type
RH-4
NA
NA

Attributes by original data submitter

Sample

source_name
RH4
cell line
RH4
antibody
Abcam (ab104245, rabbit pAb)
enrichment target
SOX8
target function
Transcription Factor
treatment
untreated

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted from ChIP-seq samples prepared following the Active Motif ChIP-IT High Sensitivity kit protocol. ATAC-seq DNA purification was performed by column (MiniElute PCR purification kit, Qiagen). Standard Illumina barcodes were introduced during library preparation

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
41839666
Reads aligned (%)
68.9
Duplicates removed (%)
28.4
Number of peaks
3814 (qval < 1E-05)

hg38

Number of total reads
41839666
Reads aligned (%)
71.4
Duplicates removed (%)
26.4
Number of peaks
4110 (qval < 1E-05)

Base call quality data from DBCLS SRA