Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Cardiovascular

Cell type

HUVEC

Primary Tissue

Umbilical Cord

Tissue Diagnosis

Normal

Sample

source_name

Human umbilical vein endothelial cells

cell type

Human umbilical vein endothelial cells

treatment

Static

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Total mRNA and genomic DNA were directly isolated from HUVEC after shear stress experiments, further DNA-histone complexes were precipitated and purified using H3K27ac antibody. ChIP and RNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

28010906

Reads aligned (%)

98.3

Duplicates removed (%)

35.2

Number of peaks

1039 (qval < 1E-05)

hg19

Number of total reads

28010906

Reads aligned (%)

97.0

Duplicates removed (%)

35.9

Number of peaks

530 (qval < 1E-05)