GSM3215439: H3K27ac osc R2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Cardiovascular
Cell type
HUVEC
Primary Tissue
Umbilical Cord
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
Human umbilical vein endothelial cells
cell type
Human umbilical vein endothelial cells
treatment
Oscillatory shear stress
chip antibody
H3K27ac (Abcam, ab4729)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total mRNA and genomic DNA were directly isolated from HUVEC after shear stress experiments, further DNA-histone complexes were precipitated and purified using H3K27ac antibody. ChIP and RNA libraries were prepared for sequencing using standard Illumina protocols