Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Cardiovascular
Cell type
HUVEC
Primary Tissue
Umbilical Cord
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
Human umbilical vein endothelial cells
cell type
Human umbilical vein endothelial cells
treatment
Static
chip antibody
H3K27ac (Abcam, ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total mRNA and genomic DNA were directly isolated from HUVEC after shear stress experiments, further DNA-histone complexes were precipitated and purified using H3K27ac antibody. ChIP and RNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
43990851
Reads aligned (%)
98.2
Duplicates removed (%)
75.1
Number of peaks
1257 (qval < 1E-05)

hg19

Number of total reads
43990851
Reads aligned (%)
97.3
Duplicates removed (%)
75.9
Number of peaks
868 (qval < 1E-05)

Base call quality data from DBCLS SRA