Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Blood
Cell type
Lymphoblastoid cell line
Tissue
blood
Lineage
mesoderm
Description
parental cell type to lymphoblastoid cell lines

Attributes by original data submitter

Sample

source_name
LCL
cell type
LCL
ID
Donor 6
antibody
H3K4me3, ab8580 (Abcam)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was isolated from B cells and LCLs with Trizol. Libraries were prepared using Kapa Hyper Prep Kit (Kapa Biosystems)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
67011942
Reads aligned (%)
77.0
Duplicates removed (%)
4.5
Number of peaks
24331 (qval < 1E-05)

hg19

Number of total reads
67011942
Reads aligned (%)
76.3
Duplicates removed (%)
4.8
Number of peaks
23926 (qval < 1E-05)

Base call quality data from DBCLS SRA