Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Blood
Cell type
B-cell (CD19+)
Tissue
Blood
Lineage
primaryCells
Description
B Cell , CD19+

Attributes by original data submitter

Sample

source_name
CD19 B cells
cell type
CD19 B cells
ID
Donor 9
antibody
H3K4me3, ab8580 (Abcam)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was isolated from B cells and LCLs with Trizol. Libraries were prepared using Kapa Hyper Prep Kit (Kapa Biosystems)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
64198035
Reads aligned (%)
89.3
Duplicates removed (%)
3.0
Number of peaks
17566 (qval < 1E-05)

hg19

Number of total reads
64198035
Reads aligned (%)
88.2
Duplicates removed (%)
3.3
Number of peaks
16177 (qval < 1E-05)

Base call quality data from DBCLS SRA