Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Blood
Cell type
B-cell (CD19+)
Tissue
Blood
Lineage
primaryCells
Description
B Cell , CD19+

Attributes by original data submitter

Sample

source_name
CD19 B cells
cell type
CD19 B cells
ID
Donor 6
antibody
H3K4me3, ab8580 (Abcam)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was isolated from B cells and LCLs with Trizol. Libraries were prepared using Kapa Hyper Prep Kit (Kapa Biosystems)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
66272324
Reads aligned (%)
71.1
Duplicates removed (%)
5.5
Number of peaks
22172 (qval < 1E-05)

hg19

Number of total reads
66272324
Reads aligned (%)
70.4
Duplicates removed (%)
5.7
Number of peaks
21051 (qval < 1E-05)

Base call quality data from DBCLS SRA