Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Epidermis
Cell type
A-375
Primary Tissue
Skin
Tissue Diagnosis
Melanoma

Attributes by original data submitter

Sample

source_name
cell culture
cell line
A375
genotype/variation
empty vector
tissue
skin
tumor stage
malignant melanoma
chip antibody
anti-AR (Santa Cruz H-280)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with Santa Cruz AR (H-280) antibody. Libraries were prepared by Active Motif.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
33463732
Reads aligned (%)
84.7
Duplicates removed (%)
11.8
Number of peaks
1695 (qval < 1E-05)

hg38

Number of total reads
33463732
Reads aligned (%)
86.3
Duplicates removed (%)
10.6
Number of peaks
1816 (qval < 1E-05)

Base call quality data from DBCLS SRA