Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EGR1

Cell type

Cell type Class
Epidermis
Cell type
A-375
Primary Tissue
Skin
Tissue Diagnosis
Melanoma

Attributes by original data submitter

Sample

source_name
cell culture
cell line
A375
tissue
skin
tumor stage
malignant melanoma
chip antibody
anti-EGR1 (Cell Signaling, 44D5)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with Cell Signaling EGR1 (44D5) antibody. Libraries were prepared by Active Motif.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
46609429
Reads aligned (%)
46.9
Duplicates removed (%)
41.5
Number of peaks
1326 (qval < 1E-05)

hg38

Number of total reads
46609429
Reads aligned (%)
48.0
Duplicates removed (%)
40.4
Number of peaks
1450 (qval < 1E-05)

Base call quality data from DBCLS SRA