Antigen

Antigen Class

TFs and others

Antigen

EGR1

Cell type

Cell type Class

Epidermis

Cell type

A-375

Primary Tissue

Skin

Tissue Diagnosis

Melanoma

Sample

source_name

cell culture

cell line

A375

tissue

skin

tumor stage

malignant melanoma

chip antibody

anti-EGR1 (Cell Signaling, 44D5)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with Cell Signaling EGR1 (44D5) antibody. Libraries were prepared by Active Motif.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

46609429

Reads aligned (%)

80.6

Duplicates removed (%)

40.4

Number of peaks

1450 (qval < 1E-05)

hg19

Number of total reads

46609429

Reads aligned (%)

80.1

Duplicates removed (%)

41.5

Number of peaks

1326 (qval < 1E-05)